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GATEWAY MANUAL CLONING


Gateway Cloning | Thermo Fisher Scientific - US
From protein expression to functional analysis, Gateway cloning technology is applicable for a variety of research areas, for truly multidisciplinary scientific studies. Circumvent the roadblocks of traditional restriction enzyme cloning—no need for ligase, subcloning steps, or the hours spent to screen countless colonies.Gateway Destination Vectors · Gateway Entry Clones · Gateway Cloning Protocols[PDF]
GATEWAY™ Cloning Technology
as described in the GATEWAY Cloning Technology Instruction Manual, but does not provide rights to synthesize primers or to perform amplification using primers containing recombination sites or portions thereof. Rights for performing amplification using primers
Gateway Recombination Cloning Technology | Thermo Fisher
Gateway recombination cloning simplifies cloning workflow The typical cloning workflow, such as traditional restriction enzyme cloning, involves many steps and can limit your cloning success. For example, certain restriction enzymes cannot be used because they might cut within your gene of interest, truncating the insert and making the gene useless for downstream expression.
Gateway Cloning Manual - DocShare
GATEWAY Cloning Technology as an Operating System for Cloning and Subcloning Genes. Once a DNA segment is cloned into the GATEWAY system, it can be easily transferred from an Entry Clone into a Destination Vector (via the LR reaction) to generate an Expression clone.
Gateway Cloning Tutorial | Geneious Prime
Thermo Fisher Scientific describe their Gateway® Technology as “a universal cloning method that takes advantage of the site-specific recombination properties of bacteriophage lambda to provide a rapid and highly efficient way to move a gene of interest into multiple vector systems.”[PDF]
GATEWAY™ Cloning Technology - uni-goettingen
GATEWAY™ Cloning Technology Version 1 Note: This product is covered by a Limited Label License (see Section 1.3) consideration paid for this product grants a Limited License with a paid up royalty to use the product pursuant to the terms set forth in the accompanying Limited Label License.[PDF]
pBAD/Thio His TOPO manual - Thermo Fisher Scientific
refer to the manual for the specific destination vector or system. All Gateway ® product manuals are available for downloading from our Web site (wwwtrogen) or by contacting Technical Service (see page 52). Glossary of Terms ® To help you understand the terminology used in the Gateway Technology, a glossary of terms is provided in
Cloning Methods - Recombination cloning systems - GATEWAY
The GATEWAY Cloning Technology is based on the site-specific recombination system used by phage l to integrate its DNA in the E. coli chromosome. Both organisms have specific recombination sites called attP in phage l site and attB in E. coli.[PDF]
Primer Design for the GATEWAY attB primers
Correct design of attB primers for amplification, cloning and expression of a gene in Gateway requires consideration of the proper placement of protein expression elements (ribosome recognition sequences, start codon, stop codons, reading frame considerations etc.) with respect to the attB recombination sites.
Plasmids 101: Gateway Cloning - Addgene
Jan 12, 2017The Gateway cloning method, developed by Invitrogen, is an in vitro version of the integration and excision recombination reactions that take place when lambda phage infects bacteria. I n vivo, t hese recombination reactions are facilitated by the recombination of attachment sites from the phage (att P) and the bacteria (att B).
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