GATEWAY MANUAL INVITROGEN
The Gateway cloning method,developed by Invitrogen,is an in vitro version of the integration and excision recombination reactions that take place when lambda phage infects bacteria. In vivo,these recombination reactions are facilitated by the recombination of attachment sites from the phage (attP) and the bacteria (attB).
Plasmids 101: Gateway Cloning - Addgene
Was this helpful?People also askWhat is Invitrogen Gateway recombination cloning?What is Invitrogen Gateway recombination cloning?Invitrogen Gateway recombination cloning technology circumvents traditional restriction enzyme based cloning limitations, enabling you to access virtually any expression system in just a few simple steps.Gateway Cloning | Thermo Fisher Scientific - USSee all results for this questionWhat are the advantages of Gateway cloning?What are the advantages of Gateway cloning?Advantages of the Gateway cloning method 1 Compatibility and flexibility. Once you generate the entry clone with your DNA sequence of interest, you can move this DNA fragment across any expression system in just one recombination step. 2 Speed. The Gateway system enables the generation of the expression construct in only 1 day, as opposed to 2+ days with traditional restriction and ligation cloning. 3 Multiple fragment cloning. You can use Gateway cloning to insert multiple DNA fragments into many vectors at once in the same tube. More items.. Plasmids 101: Gateway CloningSee all results for this questionHow does gateway technology work?How does gateway technology work?Figure 1. Gateway technology facilitates cloning of genes into and back out of multiple vectors via site-specific recombination. Once a gene is cloned into an Entry clone, you can then move the DNA fragment into one or more Destination vectors simultaneously.Reference: beta-staticersci/content/dam/fishersci/en_us/documents/progrSee all results for this questionWhat is gateway vector?What is gateway vector?Gateway vectors contain modified versions of the att sites so that scientists can easily clone in their desired DNA sequences. Gateway technology relies on the two reactions described below: The BP Reaction takes place between the attB sites flanking the insert and the attP sites of the donor vector.Reference: blogene/plasmids-101-gateway-cloningSee all results for this questionFeedback
Gateway Cloning | Thermo Fisher Scientific - US
Invitrogen Gateway recombination cloning technology circumvents traditional restriction enzyme based cloning limitations, enabling you to access virtually any expression system in just a Gateway Recombination Cloning Technology · Gateway Cloning Protocols[PDF]
pBAD/Thio His TOPO manual
For details about a particular Invitrogen destination vector or expression system, refer to the manual for the specific destination vector or system. All Gateway ® product manuals are available for downloading from our Web site (www.invitrogen) or by contacting Technical Service (see File Size: 537KBPage Count: 74[PDF]
Gateway cloning technology - Fisher Scientific
This approach, called Invitrogen™MultiSite Gateway™Pro technology, allows the mixing and matching of functional fragments in a concerted fashion to generate multi-segment constructs. MultiSite Gateway Pro technology enables you to perform pathway reconstitution, multiple gene expression and regulation, protein interaction studies, and more.File Size: 380KBPage Count: 9[PDF]
MultiSite Gateway® Pro
A brief overview about the Gateway®Technology is provided in this manual. For more details about the Gateway®Technology and the recombination reactions, refer to the Gateway®Technology with Clonase™II manual. The manual is available for downloading from www.invitrogen or by contacting Technical Service (see page 67).File Size: 1MBPage Count: 84[PDF]
GATEWAY Binary Vector (pGWB) - Shimane U
Please read the GATEWAY instruction manual (Invitrogen) for procedures, etc. Please note that our pGWB seriese are available only for basic research. If you plan to use these pGWBs for commercial research, please contact Invitrogen about licencing of GATEWAYTM. Please do not distribute to other researchers without permission.
Plasmids 101: Gateway Cloning - Addgene
The Gateway cloning method, developed by Invitrogen, is an in vitro version of the integration and excision recombination reactions that take place when lambda phage infects bacteria. In vivo, these recombination reactions are facilitated by the recombination of attachment sites from the phage (attP) and the bacteria (attB).
(PDF) Gateway Cloning Technology: Advantages and Drawbacks
Invitrogen (2006) Multisite Gateway® Pro -Using Gateway® Technology to simultaneously clone multiple DNA fragments: User manual. Single step BP/LR combined Gateway reactions Article[PDF]
Gateway Technology with Clonase II
Gateway ® destination vector of choice to generate an expression clone which may then be used in the appropriate application or expression system. 4. Convert your own vector to a destination vector. For details about a particular Life Technologies destination vector or expression system, refer to the manual for the specific destination vectorFile Size: 1MBPage Count: 74