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Molecular Cloning: A Laboratory Manual (Fourth Edition)
Description. Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years. No other manual has been so popular, or so influential. Molecular Cloning, Fourth Edition, by the celebrated founding author Joe Sambrook and new co-author, the distinguished HHMI investigator Michael Green, preserves the highly praised detail and clarity of previous editions and
Cloning - Wikipedia
Cloning is the process of producing genetically identical individuals of an organism either naturally or artificially. In nature, many organisms produce clones through asexual reproduction.Cloning in biotechnology refers to the process of creating clones of organisms or copies of cells or DNA fragments (molecular cloning)nd biology, the term refers to the production of multiple copies of
Vector (molecular biology) - Wikipedia
In molecular cloning, a vector is a DNA molecule used as a vehicle to artificially carry foreign genetic material into another cell, where it can be replicated and/or expressed (e.g.- plasmid, cosmid, Lambda phages).A vector containing foreign DNA is termed recombinant DNA four major types of vectors are plasmids, viral vectors, cosmids, and artificial chromosomes.[PDF]
pSUPER : Manual A Vector System for Expression of Short
Manual The resulting transcript of the recombinant vector is predicted to fold back on itself to form a 19–base pair stem-loop structure. Analysis indicates that the stem-loop precursor transcript is quickly cleaved in the cell to produce a functional siRNA (4).
Genome Compiler Corporation
Genome Compiler is an all-in-one free software platform for biologists. You can intuitively visualize & design DNA, import, manage and share your data.
Antibodies, ELISA Kits, Research Products - Gentaur
Find and buy Antibodies, ELISA Kits or Research Products on new gentaur online store.
MYC Recruits SPT5 to RNA Polymerase II to Promote
Introduction. Deregulated and enhanced expression of the MYC proto-oncogene contributes to the development of most human tumors (Dang, 2012, Schaub et al., 2018), and mouse models demonstrate that many cancer cells depend on high levels of MYC (Gabay et al., 2014)emic genetic inhibition of MYC in vivo results in rapid regression of established murine tumors, yet it is tolerated by
System-wide Profiling of RNA-Binding Proteins Uncovers Key
Apr 04, 2019To allow accurate quantification of RBPs associated with poly(A) RNA under different physiological conditions, we developed a cRIC approach by combining the original protocol (Castello et al., 2013) with stable isotope labeling by amino acids in cell culture (SILAC) (Figure 1A) brief, cells were grown in presence of light, medium, or heavy amino acids with incorporation efficiency >98%.
1 kb DNA Ladder | NEB
"A number of proprietary plasmids are digested to completion with appropriate restriction enzymes to yield 10 bands suitable for use as molecular weight standards for agarose gel electrophoresis. The digested DNA includes fragments ranging from 0.5-10.0 kilobases (kb). The 3.0 kb fragment has increased intensity to serve as a reference band.[PDF]
Gel Doc XR+ Imager - Bio-Rad
Gel Doc™ XR+ Imager Imaging Effortless and Accurate Gel Imaging and Analysis 7 2 9 4 9 6 3 13 17 14 11 8 6 11 2 16 9 16 9 7 5 15 14 11 7 15 12 8 6 11 2 5 12 15 11 8 6 162 5 15 11 8 2 11 2 5 2 3 18

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