SOME BIOLOGICAL TECHNIQUES IN ELECTRON MICROSCOPY
Cryogenic electron microscopy - Wikipedia
Cryogenic electron microscopy (cryo-EM) is a cryomicroscopy technique applied on samples cooled to cryogenic temperatures. For biological specimens, the structure is preserved by embedding in an environment of vitreous ice aqueous sample solution is applied to a grid-mesh and plunge-frozen in liquid ethane or a mixture of liquid ethane and propane.
Staining - Wikipedia
Staining is a technique used to enhance contrast in samples, generally at the microscopic level. Stains and dyes are frequently used in histology (microscopic study of biological tissues), in cytology (microscopic study of cells), and in the medical fields of histopathology, hematology, and cytopathology that focus on the study and diagnoses of diseases at the microscopic level.
An Introduction to the Light Microscope, Light Microscopy Techniques
Sep 02, 2021Figure 1: Basic compound microscope: Light from a source is focused onto the sample (object) using a mirror and condenser lens. Light from the sample is collected by an objective, forming an intermediate image which is imaged again by the eyepiece and relayed to the eye, which sees a magnified image of the sample.
Scanning Electron Microscope - an overview | ScienceDirect Topics
Electron dose during search and relocation phases was minimized by reducing overall frame size and keeping the pixel dwell time to an absolute minimum. 11. Optimal electron image quality was achieved by selecting an accelerating voltage appropriate to the thickness of the specimen, subject to backscatter detector sensitivity.
Total Internal Reflection Fluorescence (TIRF) Microscopy
The biological fluorophore has been inserted into hundreds of proteins, through genetic recombination, and is essentially unlimited in that potential. and electron microscopic approaches. These techniques are in some ways indirect or provide only an instantaneous representation of the processes taking place, and cannot resolve the complex
What are the Basics of Biochemical Techniques? - Biochemistry
Apr 28, 2022There has been a recent resurgence in the use of light microscopy, partly due to technical improvements that have dramatically improved the resolution far beyond the theoretical limit. For example, fluorescence microscopy has a resolution of about 10 nm, while interference microscopy has a resolution of about 1 nm. Electron Microscope