TOPO GATEWAY CLONING MANUAL
pENTR™ Directional TOPO® Cloning Kits
pENTR™ Directional TOPO® Cloning Kits Five-minute, directional TOPO® Cloning of blunt-end PCR products into an entry vector for the Gateway® System Catalog numbers K2400-20, K2420-20, K2525-20, K2535-20, K2435-20, and K2635-20 Revision Date [PDF]
pBAD/Thio His TOPO manual - Thermo Fisher Scientific
Gateway ® Entry Vectors creation of entry clones. For rapid TOPO A variety of Gateway ® entry vectors are available from Invitrogen to facilitate ® Cloning of PCR products, we recommend using the pENTR/D-TOPO® or pENTR/SD/D-TOPO ® Cloning Kits. For traditional restriction enzyme digestion and ligase-mediated cloning, use one of the other
pCR™8/GW/TOPO™ TA Cloning Kit with One Shot™ TOP10 E. coli
The pCR8/GW/TOPO TA Cloning Kit delivers simple 5 minute cloning and access to the Gateway recombination cloning vector platform. Our pCR8 TOPO TA vector contains a minimal MCS, so you sequence more of your insert and less of the vector our [PDF]
8/GW/TOPO TA Cloning Kit - Thermo Fisher Scientific
g and Gateway® technologies to facilitate 5-minute, 1-step cloning of Taq polymerase- amplified PCR products into a plasmid vector with greater than 95% efficiency. As is the case with other pCR ™ vectors (e.g. pCR 2.1-TOPO®), clones may be easily sequenced and characterized.
pENTR™/D-TOPO™ Cloning Kit, with One Shot™ TOP10
The pENTR/D-TOPO Cloning Kits utilize a highly efficient, 5-minute cloning strategy (TOPO Cloning) to directionally clone a blunt-end PCR product into a vector for entry into the Gateway System or the MultiSite Gateway System. Blunt-end PCR products clone directionally at greater than 90% efficiency
Gateway and TOPO Cloning Simulation - MacVector
Gateway and TOPO Cloning. Invitrogen market a number of kits and cloning vectors designed to simplify the cloning and subcloning of DNA fragments such as those generated by PCR amplification. Amongst the most popular are the Gateway and TOPO kits.
Plasmids 101: Gateway Cloning - Addgene
Jan 12, 2017The Gateway cloning method, developed by Invitrogen, is an in vitro version of the integration and excision recombination reactions that take place when lambda phage infects bacteria. I n vivo , t hese recombination reactions are facilitated by the recombination of attachment sites from the phage ( att P ) and the bacteria ( att B ).[PDF]
GATEWAY™ Cloning Technology
use only the purchased amount of the product to practice GATEWAY™ Cloning Technology solely for internal research purposes and only as described in the GATEWAY Cloning Technology Instruction Manual, but does not provide rights to synthesize primers or to perform amplification using primers containing recombination sites or portions thereof.[PDF]
GATEWAY™ Cloning Technology - uni-goettingen
GATEWAY™ Cloning Technology Version 1 Note: This product is covered by a Limited Label License (see Section 1.3) consideration paid for this product grants a Limited License with a paid up royalty to use the product pursuant to the terms set forth in the accompanying Limited Label License.[PDF]
GATEWAY AND TOPO CLONING - folk
GATEWAY and TOPO Cloning Chapter 23 323 o ORF and Translation Analyses – Use Vector NTI analysis tools to verify that a DNA sequence in an Expression Clone is expressed as intended before committing bench time to subsequent steps. See page 334.
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