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TOPO GATEWAY CLONING MANUAL

[PDF]
pENTR™ Directional TOPO® Cloning Kits
pENTR™ Directional TOPO® Cloning Kits Five-minute, directional TOPO® Cloning of blunt-end PCR products into an entry vector for the Gateway® System Catalog numbers K2400-20, K2420-20, K2525-20, K2535-20, K2435-20, and K2635-20 Revision Date [PDF]
pBAD/Thio His TOPO manual - Thermo Fisher Scientific
Gateway ® Entry Vectors creation of entry clones. For rapid TOPO A variety of Gateway ® entry vectors are available from Invitrogen to facilitate ® Cloning of PCR products, we recommend using the pENTR/D-TOPO® or pENTR/SD/D-TOPO ® Cloning Kits. For traditional restriction enzyme digestion and ligase-mediated cloning, use one of the other
pCR™8/GW/TOPO™ TA Cloning Kit with One Shot™ TOP10 E. coli
The pCR8/GW/TOPO TA Cloning Kit delivers simple 5 minute cloning and access to the Gateway recombination cloning vector platform. Our pCR8 TOPO TA vector contains a minimal MCS, so you sequence more of your insert and less of the vector our [PDF]
8/GW/TOPO TA Cloning Kit - Thermo Fisher Scientific
g and Gateway® technologies to facilitate 5-minute, 1-step cloning of Taq polymerase- amplified PCR products into a plasmid vector with greater than 95% efficiency. As is the case with other pCR ™ vectors (e.g. pCR 2.1-TOPO®), clones may be easily sequenced and characterized.
pENTR™/D-TOPO™ Cloning Kit, with One Shot™ TOP10
The pENTR/D-TOPO Cloning Kits utilize a highly efficient, 5-minute cloning strategy (TOPO Cloning) to directionally clone a blunt-end PCR product into a vector for entry into the Gateway System or the MultiSite Gateway System. Blunt-end PCR products clone directionally at greater than 90% efficiency
Gateway and TOPO Cloning Simulation - MacVector
Gateway and TOPO Cloning. Invitrogen market a number of kits and cloning vectors designed to simplify the cloning and subcloning of DNA fragments such as those generated by PCR amplification. Amongst the most popular are the Gateway and TOPO kits.
Plasmids 101: Gateway Cloning - Addgene
Jan 12, 2017The Gateway cloning method, developed by Invitrogen, is an in vitro version of the integration and excision recombination reactions that take place when lambda phage infects bacteria. I n vivo , t hese recombination reactions are facilitated by the recombination of attachment sites from the phage ( att P ) and the bacteria ( att B ).[PDF]
GATEWAY™ Cloning Technology
use only the purchased amount of the product to practice GATEWAY™ Cloning Technology solely for internal research purposes and only as described in the GATEWAY Cloning Technology Instruction Manual, but does not provide rights to synthesize primers or to perform amplification using primers containing recombination sites or portions thereof.[PDF]
GATEWAY™ Cloning Technology - uni-goettingen
GATEWAY™ Cloning Technology Version 1 Note: This product is covered by a Limited Label License (see Section 1.3) consideration paid for this product grants a Limited License with a paid up royalty to use the product pursuant to the terms set forth in the accompanying Limited Label License.[PDF]
GATEWAY AND TOPO CLONING - folk
GATEWAY and TOPO Cloning Chapter 23 323 o ORF and Translation Analyses – Use Vector NTI analysis tools to verify that a DNA sequence in an Expression Clone is expressed as intended before committing bench time to subsequent steps. See page 334.
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pcDNA3.1 NT-GFP-TOPO (linearized) Sequence and Map pENTR GeneBLAzer Sequence and Map pET-DEST42 Sequence and Map pDONR201 Sequence and Map pHELLSGATE Sequence and Map pEF-DEST51 Sequence and Map pSC-A-amp kan (linearized) Sequence and Map pMCS5 Sequence and Map pGEM-3Zf(+) Sequence and Map pGEM-11Zf(+) Sequence and Map pDONR221 P3-P2 Sequence and Map pDNR-Dual Sequence and Map pET-56-DEST Sequence and Map pCR4-TOPO (linearized) Sequence and Map pMAL-c5E Sequence and Map pTXB1 Sequence and Map pSEAP2-Basic Sequence and Map pF1K T7 Sequence and Map pMMB206 Sequence and Map LITMUS38 Sequence and Map pEX2 Sequence and Map pACYC177 Sequence and Map pEASY-Blunt E1 (linearized) Sequence and Map pUC57-T Sequence and Map pANT7_nHA Sequence and Map pT7CFE1-CHis Sequence and Map pESC-HIS Sequence and Map LITMUS28 Sequence and Map pANT7_cGST Sequence and Map pDONR222 Sequence and Map pDONR207 Sequence and Map pDEST15 Sequence and Map pcDNA6.2 N-EmGFP-DEST Sequence and Map pENTR3C Sequence and Map pBC SK(+) Sequence and Map pF3A WG (BYDV) Sequence and Map pKF 18k-2 Sequence and Map pTrcHis2-TOPO (linearized) Sequence and Map pHP45(omega) Sequence and Map pSTBlue-1 AccepTor Sequence and Map pCRII-TOPO (linearized) Sequence and Map T-Vector pMD19 (Simple) Sequence and Map pEASY-T1 (linearized) Sequence and Map pSP64 Poly(A) Sequence and Map YCplac111 Sequence and Map pCMV SPORT6 Sequence and Map pDEST20 Sequence and Map pcDNA3.1 nV5-DEST Sequence and Map pBluescript SK(-) Sequence and Map pBC KS(+) Sequence and Map pRSET B Sequence and Map pCoBlast Sequence and Map DNAFORM Search Engine pTA Plus (linearized) Sequence and Map pCR-Blunt II-TOPO Sequence and Map pDEST R4-R3 Vector II Sequence and Map pE-SUMOstar Kan Sequence and Map pAmCyan Sequence and Map pDEST26 Sequence and Map pET-60-DEST Sequence and Map pTV 118N Sequence and Map pUCATPH Sequence and Map pPICZ A Sequence and Map pGEM-T Easy (linearized) Sequence and Map pOSIP-TO Sequence and Map pDEST14 Sequence and Map YCplac33 Sequence and Map pEX-A Sequence and Map pRS425 Sequence and Map YIplac128 Sequence and Map pFastBac HBM-TOPO (linearized) Sequence and Map pAsRed2 Sequence and Map pHSG396 Sequence and Map pVL1392 Sequence and Map pNIC28-Bsa4 Sequence and Map pSecTag A Sequence and Map pETBlue-1 Sequence and Map pFA6a-natMX6 Sequence and Map pETcoco-1 Sequence and Map pGAPZ A Sequence and Map pLX304 Sequence and Map pOBD2 Sequence and Map pT7CFE1-NHis Sequence and Map pMetLuc2-Control Sequence and Map pCR8 GW TOPO (linearized) Sequence and Map pAcHLT A Sequence and Map pIDK Sequence and Map pTurboGFP-N Sequence and Map pLenti6.2 V5-DEST Sequence and Map pKillerRed-B Sequence and Map pETBlue-2 Sequence and Map pTYB21 Sequence and Map Try it! photoaltan9: topo ta cloning kit BP reaction is repeatedly failed. photoaltan9: topo ta cloning kit Map and pcDNA3.2/V5/GW/D-TOPO- 北京华越洋生物

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